Protease Inhibitor Cocktail
Application
Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of digesting the proteins present in the extract. TOOLS Protease Inhibitor Cocktail (100X) is a Western blot related ready-to-use concentrated stock solution reagent containing a blend of seven protease inhibitors that is to be added to cell lysis buffer to protect the integrity and functionality of native cellular proteins against degradation by multiple classes of endogenous proteases during protein extraction and sample preparation procedures. The product is supplied as a 100X concentrated stock solution in a liquid format for improved accuracy, solubility, and ease of use in comparison to traditional tablets.
Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of digesting the proteins present in the extract. TOOLS Protease Inhibitor Cocktail (100X) is a Western blot related ready-to-use concentrated stock solution reagent containing a blend of seven protease inhibitors that is to be added to cell lysis buffer to protect the integrity and functionality of native cellular proteins against degradation by multiple classes of endogenous proteases during protein extraction and sample preparation procedures. The product is supplied as a 100X concentrated stock solution in a liquid format for improved accuracy, solubility, and ease of use in comparison to traditional tablets.
Features
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Easy to use-100X solution
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EDTA included
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Economical-more cost effective
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Protease Inhibitor Component Information
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Protease Inhibitor Component
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MW
|
Protease Family Targeted
|
Inhibition Type
|
Typical Working (1X) Conc.
|
Concentration in 100X cocktail
|
|
E-64
|
357.4
|
Cysteine proteases (papain, calpain,lysosomal cathepsins)
|
Irreversible
|
1 to 20μM
|
1.5mM
|
|
AEBSF-HCI
|
239.5
|
Serine proteases (trypsin, chymotrypsin, plasmin, trypsinogen, urokinase, allikrein)
|
Irreversible
|
0.2 to 1.0mM
|
100mM
|
|
Bestatin
|
308.4
|
Amino peptidases
|
Reversible
|
1 to 10μM
|
5mM
|
|
Leupeptin
|
475.6
|
Serine and cysteine proteases
|
Reversible
|
10 to 100μM
|
2mM
|
|
Aprotinin
|
6511.5
|
Serine proteases (trypsin, chymotrypsin, plasmin, trypsinogen, urokinase, kallikrein)
|
Reversible
|
100 to 200nM
|
80μM
|
|
Pepstatin A
|
685.9
|
Aspartic acid proteases (pepsin and rennin)
|
Reversible
|
1 to 20μM
|
1mM
|
|
EDTA
|
372.2
|
Metalloproteases (thermolysin and carboxypeptidase A)
|
Reversible
|
1 to 10mM
|
1mM
|
Q1. Protease inhibitor cocktail is not dissolved in DMSO
A: We suggest you use shaker and dissovle in 4 degrees. Short sonication can also help.
Q2. Can Protease Inhibitor Cocktail dissolve in 100ml extract buffer?
A: No. We suggest you dissolve in 10ml extract buffer according to protocol.
A: We suggest you use shaker and dissovle in 4 degrees. Short sonication can also help.
Q2. Can Protease Inhibitor Cocktail dissolve in 100ml extract buffer?
A: No. We suggest you dissolve in 10ml extract buffer according to protocol.